FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195278
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16160.13519867344667935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.12072505308388509No Hit
TACTATACCACTACGACTACTGGGGCCGGGGAACCCTGGTCACCGTCTCC13320.11143851053897086No Hit
CTCCTGTAAAGCCTCAGGATTCGACTTCGGTGATTATGCTATGGCCTGGT13210.1105182225390244No Hit
CTTAAAAGGTGTCCAGTGTGAGGTACAACTGGTGGAGTCTGGGGGAACCC12210.10215196799405661No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12120.10139900508500951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG23600.054.9092563
TCAACGC23750.054.562464
CAACGCA24750.052.6487925
AACGCAG26400.049.903646
GTATCAA30000.046.4275861
CGCAGAG28750.045.3237428
ACGCAGA33150.039.3079227
TATACGG750.001529966638.424742
CCGATCG750.001535640238.395819
TATCAAC35250.038.2203522
GCAGAGT36200.036.3938149
GGTATCA18750.034.640271
TAGGACG950.004893027230.3124794
TACGTTG1453.6625293E-529.7898465
GTACATG29700.029.6444511
TACATGG29050.029.2649692
GTACTAG2752.2555469E-1028.8668941
GTATACG1500.001535015524.0557461
GTCTAGG2701.7243838E-724.0557441
TAGTACG1500.001556882923.997384
Produced by FastQC (version 0.11.5)