FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707603
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAAGACTGTCCTGTTTCAAAATTATCCGCCCTCAGGGATCCCTGACC56630.33163446070310254No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC56190.3290577493714874No Hit
GACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCCGGGCAACGAGG54380.3184580959391615No Hit
CCTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGCAGTCTGGGGGAGGCG53200.3115478246407391No Hit
GTCCTGTACTGGAAACAACTACAACATTGGAAGTTATACTGTGGGCTGGT47810.2799831108284537No Hit
GTAATAAACGGCCGTGTCCTCAGCTCTCAGGCTGGTCATTTGCAGATACA44920.26305880230943607No Hit
GTCCTCCCAAGACTGTCCTGTTTCAAAATTATCCGCCCTCAGGGATCCCT43690.25585572290514835No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCCG37800.22136292803420937No Hit
GGTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC36190.2119345070253449No Hit
GTCTTGGGAGGACCGTGCGAAATCTGTTGGTACCAGCCCACAGTATAACT34890.20432149627284563No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAAGTCCCTGAGACTCGCCTGTG34250.20057355251776907No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33220.19454170553694272No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33180.19430745905225044No Hit
CCATAGTAGTAGAACGTAAATGGTTCTGATATTGCCGATCCTCTCGCACA32630.19108656988773154No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31840.18646020181505887No Hit
GTTTATTACTGTGCGAGAGGATCGGCAATATCAGAACCATTTACGTTCTA30730.17995986186484797No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30700.17978417700132876No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29530.17293246732407944No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA27980.16385541604225337No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27410.16051740363538833No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26530.15536398097215803No Hit
CTACTATGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCTCCT25420.14886364102194716No Hit
TGGGAGGACCGTGCGAAATCTGTTGGTACCAGCCCACAGTATAACTTCCA25360.1485122712949087No Hit
GTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTACTGGAAACAACT24810.14529138213038978No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24510.14353453349519765No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24480.14335884863167844No Hit
TAGTAGTAGAACGTAAATGGTTCTGATATTGCCGATCCTCTCGCACAGTA23950.14025508270950568No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23890.13990371298246723No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23470.13744412489319824No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23450.1373270016508521No Hit
ACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCCGGGCAACGAGGT22950.13439892059219855No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22870.13393042762281396No Hit
ATAATAATCACCCTCGTCCTCAGGCTGGAGGCCCGAGATAGTCAGGGTGG22560.13211501736644876No Hit
GTGTAGACCATAGTCGCTGAAAGTGAAACCAGAGGCTGCACAGGCGAGTC22500.13176364763941031No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22500.13176364763941031No Hit
GTACTGGAAACAACTACAACATTGGAAGTTATACTGTGGGCTGGTACCAA21930.12842563523254527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.1271372795667377No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21280.12461912985629565No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC21010.12303796608462271No Hit
GATAAATATTACGCAAAGTCCGTGAGGGGCCGATTCACCGTCTCAAGGGA20980.12286228122110349No Hit
CTACTACTATGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCT20720.12133967907060365No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20670.12104687096473829No Hit
CTCCTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGCAGTCTGGGGGAGG20250.11858728287546931No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19740.11560064019564267No Hit
GGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCT19690.11530783208977731No Hit
AAGTTATACTGTGGGCTGGTACCAACAGATTTCGCACGGTCCTCCCAAGA19110.11191125806173918No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19010.11132564185000846No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18910.11074002563827776No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18760.10986160132068169No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG18260.10693352026202813No Hit
TTCTACTACTATGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCATCGT18250.10687495864085506No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT18070.10582084945973977No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA17800.10423968568806684No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAACTGATAAATATTA17660.10341982299164384No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA17630.10324413812812462No Hit
ATATTTATCAGTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT17560.10283420677991313No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17160.10049174193299028No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)