FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1707603
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAAGACTGTCCTGTTTCAAAATTATCCGCCCTCAGGGATCCCTGACC51060.29901563770970185No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC49230.28829886103502983No Hit
CCTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGCAGTCTGGGGGAGGCG47730.2795146178590691No Hit
GACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCCGGGCAACGAGG47630.2789290016473384No Hit
GTCCTGTACTGGAAACAACTACAACATTGGAAGTTATACTGTGGGCTGGT43720.25603140776866756No Hit
GTAATAAACGGCCGTGTCCTCAGCTCTCAGGCTGGTCATTTGCAGATACA40920.23963415384020756No Hit
GTCCTCCCAAGACTGTCCTGTTTCAAAATTATCCGCCCTCAGGGATCCCT38770.22704340528799724No Hit
GTCTTGGGAGGACCGTGCGAAATCTGTTGGTACCAGCCCACAGTATAACT34050.19940232009430767No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCCG33960.19887526550374998No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32390.1896810909795778No Hit
GGTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC31270.18312218940819383No Hit
CCATAGTAGTAGAACGTAAATGGTTCTGATATTGCCGATCCTCTCGCACA29400.1721711662488295No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAAGTCCCTGAGACTCGCCTGTG28850.16895027708431057No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28830.16883315384196443No Hit
GTTTATTACTGTGCGAGAGGATCGGCAATATCAGAACCATTTACGTTCTA28670.16789616790319528No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26880.15741363771321554No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC26750.15665233663796563No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26640.15600815880506183No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26560.15553966583567727No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25900.15167459883825457No Hit
TGGGAGGACCGTGCGAAATCTGTTGGTACCAGCCCACAGTATAACTTCCA23940.1401965210883326No Hit
CTACTATGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCTCCT23540.13785405624140976No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22770.13334481141108326No Hit
GTCAGGGACCGTGGGACAGAAGGTCACCCTGTCCTGTACTGGAAACAACT22440.13141227791237192No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22410.13123659304885268No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22220.13012392224656433No Hit
TAGTAGTAGAACGTAAATGGTTCTGATATTGCCGATCCTCTCGCACAGTA21980.12871844333841062No Hit
ATAATAATCACCCTCGTCCTCAGGCTGGAGGCCCGAGATAGTCAGGGTGG21710.1271372795667377No Hit
GTGTAGACCATAGTCGCTGAAAGTGAAACCAGAGGCTGCACAGGCGAGTC21280.12461912985629565No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21120.1236821439175265No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20970.12280371959993042No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20810.12186673366116128No Hit
CTACTACTATGGTGTGGACGTCTGGGGCCAAGGGACCACGGTCATCGTCT20070.11753317369435402No Hit
GTACTGGAAACAACTACAACATTGGAAGTTATACTGTGGGCTGGTACCAA20050.11741605045200787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19810.11601057154385416No Hit
GATAAATATTACGCAAAGTCCGTGAGGGGCCGATTCACCGTCTCAAGGGA19720.11548351695329652No Hit
ACCTAGGACGGTCAACTTGGTCCCCCCGCCGAACACCCGGGCAACGAGGT19610.11483933912039275No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19390.11355098345458517No Hit
CTCCTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGCAGTCTGGGGGAGG18750.10980303969950862No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18670.10933454673012404No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18640.10915886186660481No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18520.10845612241252797No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18380.10763625971610498No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18370.10757769809493191No Hit
ATATTTATCAGTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT17950.1051181100056629No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17760.10400543920337456No Hit
GGCCTGGACCCCTCTCCTCCTCCAGCTTCTCACCCTCTGCTCAGGGTCCT17700.10365406947633612No Hit
AAGTTATACTGTGGGCTGGTACCAACAGATTTCGCACGGTCCTCCCAAGA17660.10341982299164384No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17530.10265852191639392No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT17170.10055030355416335No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC17150.10043318031181721No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17110.10019893382712491No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)