FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004344
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGTCTTTAGGCTGTCCATTTGCAGATACA10620.1057406625618314No Hit
GTCTTTAGGCTGTCCATTTGCAGATACAGGGAGTTCTTTGAATCGTCTCT10310.1026540707168062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13100.046.7075584
ATCAACG14350.043.1405943
GTATCAA15250.042.527171
CAACGCA14400.041.991015
AACGCAG15650.038.6370936
TATCAAC16900.037.0571562
ACGCAGA16800.036.4207767
CGCAGAG16850.036.3127028
GTACATG24050.031.161051
TCCGCTA2003.4640289E-728.7938358
TACATGG25400.028.340392
CCGACCG2853.5106495E-1027.7835259
ACATGGG26100.026.7528933
GGTATCA9650.026.1357271
TACACGG11050.024.1034375
AATCCGC2101.6433953E-523.9948646
GTATAGA4002.910383E-1123.4194771
ATACACG11850.023.0836664
CCGAATA1600.002267229822.4951869
TGCACCG5150.022.3641455
Produced by FastQC (version 0.11.5)