FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001348
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTATACTGCTTGGACCCGGAGTATGTCCTTCCCAGCCACTCAAGGCC32400.32356383594914057No Hit
GTATAATGATTATGCCGTATCTGTGAAAGGTCGAATAACCATCATTTCAG29200.29160691388008964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25120.2508618382420497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23510.2347835118260585No Hit
CTTTTAGACAGCTCCAAGAATAGGAACTACTTAGCTTGGTACCAGCAGAA22660.22629495440146682No Hit
TTATTACTGTCAGCAATTTTATAGTAATTTTCGTTTATTCACTTTCGGCC20950.20921797417081775No Hit
GTTTATTACTGTCAGCAATTTTATAGTAATTTTCGTTTATTCACTTTCGG18080.18055660969013768No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17820.1779601097720273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.16308016793362545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15060.1503972644874709No Hit
CCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCCAGGAC14950.14929874529134726No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14850.1483000914766894No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTA14120.14100991862968718No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13990.139711668670632No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13490.1347183995973428No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12600.12583038064688798No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT12490.12473186145076437No Hit
GTCTAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA12440.12423253454343545No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGA12340.12323388072877761No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10520.1050583813020049No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10490.10475878515760755No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10220.10206241985803138No Hit
AAAATATACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGCTGCAGGGAGA10030.10016497761018148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)