FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001348
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATTATACTGCTTGGACCCGGAGTATGTCCTTCCCAGCCACTCAAGGCC30540.30498887499650473No Hit
GTATAATGATTATGCCGTATCTGTGAAAGGTCGAATAACCATCATTTCAG30140.30099425973787336No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24030.23997651166227923No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23050.23018970427863242No Hit
TTATTACTGTCAGCAATTTTATAGTAATTTTCGTTTATTCACTTTCGGCC20050.20023008983889715No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGTGA18010.17985755201987721No Hit
CTTTTAGACAGCTCCAAGAATAGGAACTACTTAGCTTGGTACCAGCAGAA17300.17276710993580652No Hit
GTTTATTACTGTCAGCAATTTTATAGTAATTTTCGTTTATTCACTTTCGG16900.16877249467717517No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16510.1648777448000096No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15340.15319349516851286No Hit
CCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCCAGGAC14720.14700184151763424No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTA13300.13282095734949287No Hit
GTCTAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA13150.1313229766275061No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGTTGCTGATGGTGAGAGT12970.129525399761122No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12570.12553078450249064No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12450.12433239992490122No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11970.1195388616145436No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11870.11854020779988575No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT11850.11834047703695419No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10590.10575743897226539No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10440.10425945825027862No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10280.10266161214682608No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)