FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354857
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38040.16153847133817467No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45800.075.3395841
TCAACGC53750.062.6927954
ATCAACG54300.062.1903883
CAACGCA56000.060.172615
AACGCAG57750.058.596066
GGTATCA21600.056.695471
TATCAAC60550.055.8899772
ACGCAGA65150.051.7194637
CGCAGAG72050.046.7664578
GCAGAGT85050.039.787459
GTACATG69750.029.847421
GTATACG2452.1154847E-929.4026931
ACATGGG70000.027.1553963
TACATGG76350.026.5944082
CAGAGTA80800.025.2172459
GTATTAG14100.022.4795061
TATTAGG12300.021.6594222
TAGCGCG3004.8724905E-721.6008834
TATACTG13450.021.413065
CATGGGG51800.018.348244
Produced by FastQC (version 0.11.5)