FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354857
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.14748241612972676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44700.075.059521
TCAACGC52450.062.8759044
ATCAACG52750.062.518323
TATCAAC58550.056.6941572
CAACGCA58850.055.9157265
AACGCAG60150.054.8269546
GGTATCA22400.052.392241
ACGCAGA65350.050.574477
CGCAGAG71000.046.4484678
GCAGAGT85050.038.5977979
GTACATG68350.028.0201511
ACATGGG67700.026.4834733
TACATGG71000.026.266712
CAGAGTA78700.023.783229
TACCGTA2802.5056215E-723.1445487
TATACGG2557.3389216E-519.7661512
TATACCG2257.195463E-419.2014055
GTACCGT2701.1381877E-418.6680326
CATGGGG52200.018.208234
TAGTACG5951.6370905E-1118.152594
Produced by FastQC (version 0.11.5)