FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756666
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA36640.48422950152378985No Hit
GTGTATTACTGTGCGGGGGAGTACTTCGATTTTTGGAGTGGTTTCCGCCT26130.3453307007318949No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25270.3339650519515876No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAAGTGTTGGTG19770.26127776324032004No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18100.23920725921344424No Hit
GTATTACTGTGCGGGGGAGTACTTCGATTTTTGGAGTGGTTTCCGCCTTG17400.22995614974110112No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17160.22678434077915485No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG17150.22665218207240712No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16240.214625739758361No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA15530.20524247157927014No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCA15190.2007490755498463No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15070.1991631710688732No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15010.19837021882838662No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14570.19255523573148522No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14470.19123364866400763No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14450.19096933125051213No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14350.1896477441830345No Hit
GGTTATTACTATGTCTCCTGGTATCGACAACACCCAGGCAAAGCCCCCAA13780.18211469789841225No Hit
CTCCTGGAGAGTCGATCACCATCTCCTGCACTGGAACCAACAATGACGTT13760.18185038048491672No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13730.18145390436467346No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGCTCTC13660.18052879341743913No Hit
CTCCTGCACTGGAACCAACAATGACGTTGGTGGTTATTACTATGTCTCCT13370.17669619092175412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.1748459690272855No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13030.1722027948923303No Hit
GATTATTACTGCAGCTCATATACAAGCACCAACACTTGGGTGTTCGGCGG12990.17167416006533925No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12490.1650662247279513No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG12420.164141113780717No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12200.16123362223226628No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12020.15885476551080663No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG11820.15621159137585144No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11730.1550221630151216No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11660.1540970520678873No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11370.15026444957220228No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG11340.14986797345195899No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAAGTGTTGGTGC10970.14497810130229188No Hit
TTTAGAAGGTGTCCAGTGTGAGAAACAACTGGTGGAGTCTGGGGGACGCT10850.1433921968213188No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT10780.14246708587408446No Hit
GTCCACATAGTCCTTCTCCCTTCCATCTTGTTTTATGGTGGCCACCCACT10320.13638778536368754No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10250.13546267441645324No Hit
CCATAAAACAAGATGGAAGGGAGAAGGACTATGTGGACTCTGTGAAGGGC10170.13440540476247115No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10100.13348029381523685No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA10090.13334813510848909No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10050.13281950028149803No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTTTTGGATGAGCTGGG9990.1320265480410115No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9960.13163007192076823No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9820.1297798500262996No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9810.12964769131955184No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9760.12898689778581302No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTTTTGG9660.12766531071833545No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9660.12766531071833545No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9640.12740099330483992No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9620.1271366758913444No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9570.1264758823576056No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9500.1255507714103713No Hit
GTACATGGGAGGTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTT9480.12528645399687577No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9430.12462566046313697No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9330.12330407339565938No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9310.12303975598216385No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9220.12185032762143404No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9180.12132169279444299No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9160.12105737538094746No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9140.12079305796745196No Hit
GTAATAACCACCAACGTCATTGTTGGTTCCAGTGCAGGAGATGGTGATCG9140.12079305796745196No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8970.11854635995274004No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8910.1177534077122535No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8820.11656397935152366No Hit
TTACTATGTCTCCTGGTATCGACAACACCCAGGCAAAGCCCCCAAACTCA8710.1151102335772983No Hit
GTTTTATGGTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC8700.11497807487055056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8680.11471375745705503No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8580.11339217038957743No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8530.11273137685583864No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAAGCTAC8430.11140978978836104No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8280.10942740918714465No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8240.10889877436015363No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8230.10876661565340587No Hit
GCTCATATACAAGCACCAACACTTGGGTGTTCGGCGGAGGGACCAGGCTG8200.10837013953316257No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8180.10810582211966707No Hit
GCTGTGTATTACTGTGCGGGGGAGTACTTCGATTTTTGGAGTGGTTTCCG8040.10625560022519842No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT8040.10625560022519842No Hit
TATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG7790.10295163255650445No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7690.10163004548902686No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7640.10096925195528807No Hit
ATTTTAGAAGGTGTCCAGTGTGAGAAACAACTGGTGGAGTCTGGGGGACG7630.10083709324854032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4700.095.022731
GTATCAA12500.074.3384251
ATACGGT300.00198994971.995286
TATCAAC15400.060.3157042
ATCAACG15300.060.2393043
TCAACGC15500.059.4541634
CAACGCA16050.057.4167945
AACGCAG16200.056.885166
CGCAGAG20800.044.3047878
GCAGAGT21350.042.826239
GTACATG15150.038.0374151
TACATGG15550.036.581212
ACGCAGA25550.036.0680857
ACATGGG16150.035.2221573
CAGCCGT15300.033.880139
GTAATAC16200.033.7934271
CATGGGG10650.033.1245844
GGTTTAT8750.032.9295351
ATTGAGT8500.032.1861236
CTAGACT900.003753917831.9979024
Produced by FastQC (version 0.11.5)