FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786736
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20270.2576467836733034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15860.20159240202558418No Hit
GTAATAGACGGCCGTGTCGTCAGATGTCAAACTGCTCAGCTCCATGTAGG13760.1748998393361941No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11630.14782595432266987No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11540.14668198735026744No Hit
TTACTACTGCATGCAAGCTCTACAAACTCCTCCGTGGACGTTCGGCCAGG11470.1457922352606211No Hit
CTACAAACTCCTCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAA11470.1457922352606211No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA11440.14541091293648695No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10440.13270016879868216No Hit
CTAATGGATACAACTATTTGGATTGGTACCTTCAGAAGCCAGGGCAGTCT9980.12685322649529193No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9540.12126049907465783No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9540.12126049907465783No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9180.1166846311850481No Hit
GTCTATTACTGCGCGAGAGGCCAATCCGGGGTGGTTCTCTTTGGTGAAAA9150.11630330886091395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8990.11426958979886517No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8610.10943950702649936No Hit
CAGTAGTAAATCCCAACATCCTCAGCCTCCACTCTGGTGATTTTCAGTGT8150.10359256472310914No Hit
GTAGTAAATCCCAACATCCTCAGCCTCCACTCTGGTGATTTTCAGTGTAA7980.10143173821968232No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7960.10117752333692624No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7900.10041487868865794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12000.055.2235533
TCAACGC12350.053.0685164
GTATCAA13500.051.2283631
TATCAAC14250.049.0345542
CAACGCA14400.045.5136265
AACGCAG15000.044.159186
CGCAGAG15750.042.0563628
GGTATCA6100.040.153381
ACGCAGA17850.037.108567
ACACCGG1051.9304844E-434.2850766
AAATCCG1708.2876795E-833.8924985
AATCCGC1851.7459388E-731.1345586
GTAAGAC1902.1971937E-730.3287263
TACACGG4900.029.3872096
GTAATAG8850.029.304361
ATAGACG7400.029.197934
GTACACG4950.029.099625
GACGGCC7450.027.05997
GCAGAGT24600.026.9263299
ACACGGG5400.026.6661727
Produced by FastQC (version 0.11.5)