FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488007
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9160.18770222558282976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.18421866899450212No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8540.1749974897901055No Hit
GTATAAAAATTCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATTA8030.1645468200251226No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTGGA6620.1356537918513464No Hit
TTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT5800.11885075419000136No Hit
ATGTATTACTGTGCGGGGGCATTGTATTACTTTGATACTAGTCACCTCCC5680.11639177306882893No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5370.1100394051724668No Hit
GTATTACTTTGATACTAGTCACCTCCCCCCACACGGAACGGGACATGATG5110.10471161274325984No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG4970.10184280143522531No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC4960.10163788634179427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12200.063.732674
ATCAACG12250.063.4790423
CAACGCA12700.061.7903945
AACGCAG13300.059.0028576
CGCAGAG15800.049.6669658
ACGCAGA16300.048.1434367
GTATCAA17250.047.2098581
GGTATCA8950.045.0929261
TATCAAC17400.044.690712
GCAGAGT18250.043.393839
GTCCATA1901.364242E-1037.9306071
GTCTAAC850.00282206833.9144251
GTACACG2354.589492E-827.5722925
GGGCGCG1050.00797232727.4264079
GTACATG10550.026.6413081
TACACGG2207.9434903E-726.179756
GTCTAGG1959.15738E-625.870621
TCTACAC3358.185452E-1125.791653
GTCTAAG2259.577707E-725.6242311
GCATAGT1701.0729144E-425.435821
Produced by FastQC (version 0.11.5)