FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488007
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8440.1729483388557951No Hit
GTATAAAAATTCCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATTA8380.1717188482952089No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7780.15942394268934668No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTGGA6570.1346292163841912No Hit
TTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT5980.12253922587176004No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5800.11885075419000136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5350.10962957498560472No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5320.10901482970531161No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5220.10696567877100124No Hit
ATGTATTACTGTGCGGGGGCATTGTATTACTTTGATACTAGTCACCTCCC5210.1067607636775702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)