FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898264
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACTACTGTGCGAGAGGACTGTGGACGGACTGGGGCCAGGGAACCCT38950.4336141713349305No Hit
GTAGTATATAGCCGTGTCCTCGTCTCTCAGGCTGTGCATTTGCAGATATA37530.4178059011604607No Hit
GCTATATACTACTGTGCGAGAGGACTGTGGACGGACTGGGGCCAGGGAAC20440.2275500298353268No Hit
GTATATAGCCGTGTCCTCGTCTCTCAGGCTGTGCATTTGCAGATATAGTG19250.21430225412573584No Hit
GTCCCAGGCTGTGCAGTAATAGTCAGCTTCGTCCTCAGGTTGGAGCCCAG18870.21007187196637067No Hit
GCCTGGGACAGCGACCTCAGTGCTTGGCTGTTTGGCGGAGGGACCAGGCT18550.2065094448848No Hit
TCCAGAGACAATGCCAAGAACTCACTATATCTGCAAATGCACAGCCTGAG15990.17801002823223464No Hit
CATTAGCACTTCTGGTACCCCCACATATTATGCAGATTCTGTGAAGGGCC13380.1489539823481738No Hit
GTCTCTGGATATGCTGAATCGGCCCTTCACAGAATCTGCATAATATGTGG12440.13848935279605995No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11910.13258908294220853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.1299172626310305No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10390.11566755430474783No Hit
GTGCTGGGTTTTCCTTGTTGGTATTTTACAAGGTGTCCAGTGTGAGGTGC10280.1144429699954579No Hit
TTTACAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT9760.10865402598790555No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9750.10854270014160647No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9720.10820872260270922No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9630.10720678998601747No Hit
ATACTACTGTGCGAGAGGACTGTGGACGGACTGGGGCCAGGGAACCCTGG9520.10598220567672756No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9080.10108386843956788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)