FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294149
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG23750.18351828112528001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22950.17733661270842846No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21930.16945498547694277No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA18230.1408647690490044No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA14110.10902917670221898No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG13930.10763830130842739No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29850.070.432161
GGTATCA12900.060.279071
ATCAACG34650.059.6363643
TCAACGC35450.058.4936524
TATCAAC37450.055.3698272
CGCAGAG40900.050.875318
ACGCAGA41500.050.3132557
CAACGCA46800.044.6153875
AACGCAG47750.043.727756
GCAGAGT47550.043.0031559
TACATGG38850.036.6949842
GTACATG40100.035.7306751
ACATGGG41200.034.2524263
ATACCGT1402.8802873E-530.8571416
CATGGGA20000.029.5199994
GCCCATA13050.028.137931
GTATACG1358.417437E-426.6666661
GTACGGA2259.677769E-725.66
TCCGCGC3109.313226E-1025.5483888
CATGGGG24400.025.377054
Produced by FastQC (version 0.11.5)