FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902345
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGCTGCGTCGGTGAAAGGCAGGTTCACCATTTCCAGAGATGATTC28630.3172844089566629No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28260.31318398173647555No Hit
GCATATACTGTCGCGTAATTATTACCTTTGCCTCTAATACGGCCAACCCA22830.25300744172129286No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGATGCTG22410.24835290271459365No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT20330.2253018523957023No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA20270.22463691825188814No Hit
GTACTAGACATTGGGATGGGGTCGACCGTTACGAAAATTCAATCGATGCT18050.20003435493076374No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17810.19737461835550701No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACAGGATCCAGCAGTGACATT16950.1878438956275039No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG16860.18684649441178264No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA16830.18651402733987554No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGT16280.18041879768824562No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC16010.17742659404108185No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT15820.17532096925233698No Hit
GTCTAGTACAATAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATT15820.17532096925233698No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15610.17299369974898735No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT15130.16767422659847397No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14680.16268722051986767No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14630.16213310873335587No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14530.16102488516033225No Hit
GTATTATTGTACTAGACATTGGGATGGGGTCGACCGTTACGAAAATTCAA13630.15105087300311965No Hit
AGTATATGCTGCGTCGGTGAAAGGCAGGTTCACCATTTCCAGAGATGATT13540.1500534717873984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13460.1491668929289795No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGATGCTGC13310.14750455756944406No Hit
GTTTGGAGGGTGTGGTGGTCTCCATTCCCGCCTTGACGGGGCTGCTATCT13210.14639633399642044No Hit
TATACAAGCAGCATCACTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT12990.14395824213576847No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12980.14384741977846613No Hit
CTCCTGCACAGGATCCAGCAGTGACATTGGTGGTTATAAGTATGTCTCCT12970.14373659742116374No Hit
GCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCC12810.14196343970432596No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG12550.13908205841446453No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12480.138306301913348No Hit
GATTATTACTGCAGCTCATATACAAGCAGCATCACTTGGGTGTTCGGCGG12390.13730890069762672No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG12330.13664396655381256No Hit
CAATAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATA12110.1342058746931606No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11650.12910804625725195No Hit
GTGTGGTGGTCTCCATTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11530.1277781779696236No Hit
TCTCTGGGCTCCAGGCTGACGACGAGGCTGATTATTACTGCAGCTCATAT11520.12766735561232123No Hit
GCTATGCACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGGTTGG11450.1268915991112047No Hit
ACAGTATATGCTGCGTCGGTGAAAGGCAGGTTCACCATTTCCAGAGATGA11320.125450908466274No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11010.12201541538990077No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10870.12046390238766769No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA10840.1201314353157606No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10840.1201314353157606No Hit
GGATCCAGCAGTGACATTGGTGGTTATAAGTATGTCTCCTGGTACCAACA10720.11880156702813226No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC10610.11758252109780626No Hit
ATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACGCCG10570.11713923166859683No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10470.11603100809557321No Hit
GGTAATAATTACGCGACAGTATATGCTGCGTCGGTGAAAGGCAGGTTCAC10450.11580936338096849No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10180.1128171597338047No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC10090.11181975851808344No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10060.11148729144617635No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10010.11093317965966455No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9970.11048989023045509No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9950.11026824551585038No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9810.1087167325136173No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG9790.10849508779901258No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT9630.10672193008217479No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9540.10572452886645353No Hit
ATAATACACGGCCGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACG9520.10550288415184879No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9420.10439466057882515No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCAGA9340.10350808172040628No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9270.10273232521928974No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9270.10273232521928974No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9210.10206739107547556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6100.075.762111
GTATCAA17950.067.182151
TCAACGC18600.065.027464
ATCAACG18700.064.2947163
CAACGCA19750.061.6055765
TATCAAC20350.058.4517972
AACGCAG21450.057.058726
CGCAGAG23900.050.908378
TCGAAAC605.1183393E-447.996453
GAATACG3200.047.246519
GTGTAGA4100.045.7921871
GCAGAGT27400.044.405489
GTGCTAG3700.040.9844861
GTACATG21650.039.6909031
ACGCAGA31000.039.4809537
TACATGG22850.037.2283822
ACATGGG23300.036.7698143
CTAGAGA4900.035.2627034
TCAACGG900.003754450231.9976353
GCTAGAG5700.030.3135493
Produced by FastQC (version 0.11.5)