FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151215
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC81690.3797388917425734No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG62430.2902080917063148No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC50570.23507645679302164No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50560.23502997143474733No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG48550.22568641442161755No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45130.20978842189181462No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT44650.20755712469464932No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA41700.19384394400373742No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA40380.18770787671153277No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT38910.18087452904521398No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA37860.17599356642641484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37400.17385523994579807No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT35160.1634425196923599No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA34610.16088582498727463No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT34270.1593053228059492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC34190.15893343993975498No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT33030.1535411383799388No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31970.14861369040286534No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA31070.14443000815818038No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30310.1408971209293353No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30130.1400603844803983No Hit
GTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA29980.13936310410628414No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA29710.13810799943287863No Hit
GCTCATATACAAGCACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTG29470.13699235083429595No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28610.1329946100227081No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA28610.1329946100227081No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTGC28410.13206490285722255No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG28110.13067034210899423No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG27700.12876444241974885No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTG27640.12848553027010318No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTC26590.12360456765130404No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG26440.12290728727718987No Hit
TCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCT26260.12207055082825288No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25820.12002519506418466No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT25460.11835172216631068No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT25160.11695716141808235No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24940.11593448353604822No Hit
TATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG24910.1157950274612254No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT23550.10947301873592366No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA22490.10454557075885024No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC22170.10305803929407334No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC21980.10217481748686208No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA21880.10170996390411931No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA21740.1010591688882794No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.079.411321
GTATCAA44900.074.086841
ATCAACG53450.061.5650253
TCAACGC54300.060.733914
TATCAAC56600.058.520362
CAACGCA56700.057.782185
AACGCAG59200.055.0987936
ACGCAGA68250.048.003667
CGCAGAG68750.047.6545378
GCAGAGT90200.036.0767029
TATTGAG34500.031.7241555
GTACATG60850.029.4634861
TACATGG61300.027.9564532
ACATGGG61800.027.6137563
TTATTGA40100.027.2938524
ATTGAGT27800.027.1963166
CAGAGTA73450.027.05289
GGTTTAT30950.026.0557021
GTAATAC19400.024.124431
CATGGGG42900.024.001834
Produced by FastQC (version 0.11.5)