FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314960
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCTATTCATTTGCAGATACA45620.19706604001797004No Hit
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGA42440.1833293015862045No Hit
GTGTATTACTGTGCGAGAGATGACTACACTGACTACTGGGAAGACACCTA41410.1788799806476138No Hit
ACCATACATTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGA36990.15978677817327297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34440.14877146905346098No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30470.13162214465908698No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26820.11585513356602273No Hit
GTACTACCATACATTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCC26710.11537996336869752No Hit
GTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAACGGTCTGGAGTGGG25590.11054186681411342No Hit
ATGTATGGTAGTACCACTCCCACTAATGTATGAAACCCACTCCAGACCGT23670.10224798700625498No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23390.10103846286760895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45350.076.090571
GGTATCA18700.075.1217351
TCAACGC56400.060.6416934
ATCAACG57000.060.0033573
TATCAAC59050.058.0422022
CAACGCA59500.057.603225
AACGCAG61850.055.7602166
CGCAGAG69000.049.564788
ACGCAGA74750.045.9447447
GCAGAGT80900.042.3630379
GTACATG59750.030.0216141
TACATGG60500.029.1586572
ACATGGG60800.028.6594963
CAGAGTA83650.026.7684429
CATGGGA30350.024.4362934
GAATACG4500.023.9997869
GTATATA14250.023.7605021
TATATCG2654.01551E-621.7370665
ACCGTAT2053.8441853E-421.0729858
TACACGG14050.020.498046
Produced by FastQC (version 0.11.5)