FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925800
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTTCACCTCTCAGACTGGTCATTTGCAGATACA49270.5321883776193562No Hit
GTCTATTACTGTGCGAGAGAGGGGGAATATCAAAGTACCAGCTCGCACTT44370.4792611795204148No Hit
GTATTATGCAGAGTCCGTGGAGGACCGATTTACCATCTCCAGAGACAATT21650.23385180384532298No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGCAGTCTGGGGGAGGCG20820.22488658457550226No Hit
GCATAATACTTATTACTTCCATCATACGAGATAACTGCCACCCACTCCAG18990.2051198963058976No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18480.19961114711600775No Hit
GTCTGGGGGAGGCGTGGTCCACCCTGGGAGGTCTCTGAGACTCTCCTGTG16180.17476776841650465No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15650.16904298984661914No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15480.16720674011665587No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14680.1585655649168287No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13900.15014041909699719No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13400.1447396845971052No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13280.14344350831713115No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13060.14106718513717864No Hit
TCCACGGACTCTGCATAATACTTATTACTTCCATCATACGAGATAACTGC12660.13674659753726506No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12380.13372218621732554No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12130.13102181896737958No Hit
GTCCGTGGAGGACCGATTTACCATCTCCAGAGACAATTCCAAGAGCACAC11950.12907755454741845No Hit
CTCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTCGCAATGCT11680.12616115791747678No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11580.12508101101749838No Hit
GACCTAGGACGATCACCTTCGTCCCTCCGCCGAATGCCGCACTCAGGCTG10620.11471160077770577No Hit
GTCTGAGAGGTGAAGACACGGCTGTCTATTACTGTGCGAGAGAGGGGGAA10470.11309138042773818No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCGCAATGCTATGCACTGGA10200.1101749837977965No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9870.10661049902786779No Hit
CCCTGGGAGGTCTCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA9840.10628645495787427No Hit
GCTATGCACTGGATCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9810.10596241088788076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.10466623460790667No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9550.10315402894793692No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9370.1012097645279758No Hit
CTGTATCTGCAAATGACCAGTCTGAGAGGTGAAGACACGGCTGTCTATTA9330.10077770576798445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)