FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925800
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTTCACCTCTCAGACTGGTCATTTGCAGATACA47040.508101101749838No Hit
GTCTATTACTGTGCGAGAGAGGGGGAATATCAAAGTACCAGCTCGCACTT43010.4645711816807086No Hit
GTATTATGCAGAGTCCGTGGAGGACCGATTTACCATCTCCAGAGACAATT21710.23449989198531No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20450.22089004104558219No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGCAGTCTGGGGGAGGCG19710.21289695398574204No Hit
GCATAATACTTATTACTTCCATCATACGAGATAACTGCCACCCACTCCAG18500.19982717649600343No Hit
GTCTGGGGGAGGCGTGGTCCACCCTGGGAGGTCTCTGAGACTCTCCTGTG15750.17012313674659751No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15290.16515446100669692No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15010.1621300496867574No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13860.14970836033700582No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13110.14160725858716786No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12970.1400950529271981No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12930.13966299416720676No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12900.13933895009721323No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12410.13404623028731907No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11990.12950961330740982No Hit
GTCCGTGGAGGACCGATTTACCATCTCCAGAGACAATTCCAAGAGCACAC11840.1278893929574422No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11660.1259451285374811No Hit
TCCACGGACTCTGCATAATACTTATTACTTCCATCATACGAGATAACTGC11420.12335277597753294No Hit
CTCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTCGCAATGCT10980.118600129617628No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTCGCAATGCTATGCACTGGA10890.11762799740764743No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10650.11503564484769929No Hit
CTGTATCTGCAAATGACCAGTCTGAGAGGTGAAGACACGGCTGTCTATTA10390.11222726290775545No Hit
GTCTGAGAGGTGAAGACACGGCTGTCTATTACTGTGCGAGAGAGGGGGAA10380.11211924821775761No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10380.11211924821775761No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10170.10985093972780298No Hit
CCCTGGGAGGTCTCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA10080.10887880751782242No Hit
GACCTAGGACGATCACCTTCGTCCCTCCGCCGAATGCCGCACTCAGGCTG9940.10736660185785268No Hit
GCTATGCACTGGATCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9430.10185785266796285No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9330.10077770576798445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12000.047.414184
ATCAACG12200.046.6368943
CAACGCA12750.044.625115
AACGCAG13400.042.981676
GTATCAA15100.042.933711
TATCAAC13650.042.2127042
CGCAGAG13700.042.0404668
CAGCCGT17850.038.7175339
TAGACAG19550.037.208065
ATAGACA20100.035.114984
CTGTGCG21950.035.0932779
AATAGAC19850.034.8315733
GTAATAG21000.033.61551
GAATACG1501.1980301E-633.5955289
GTCTATT23050.033.438411
ACTGTGC26050.032.335258
TAATAGA21400.032.3104742
TCTATTA24550.032.2720872
AGACAGC23350.031.7576566
TACTGTG26600.031.3960117
Produced by FastQC (version 0.11.5)