FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890970
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATAGA46180.5183115031931491No Hit
GTATATTACTGTGCGAGAGGGGGGTCAAATTCTTTGGTGTTAATTCCTAT37040.41572667991065915No Hit
TCATAATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGGAGCAGGCT36960.4148287821138759No Hit
GTATTATGATACCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCA35780.4015847896113225No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22630.25399283926507066No Hit
ATGATACCCCGCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGA21990.2468096568908044No Hit
GACCAGGGAGAGCCCCTAAACTCCTTCTCTATGCAGCATCCAGATTGCAA20520.2303107848749116No Hit
GGGTATCATAATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGGAGC19080.21414862453281255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17840.2002312086826717No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGTGGAGTCTGGGGGAGGCG17280.19394592410518874No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16800.18855853732448905No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16630.1866505045063246No Hit
ATATACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATAGAGGG16480.18496694613735593No Hit
GCTGTATATTACTGTGCGAGAGGGGGGTCAAATTCTTTGGTGTTAATTCC14400.1616216034209906No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14270.1601625195012178No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14220.15960133337822824No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13610.1527548626777557No Hit
GCTATGCACTGGGTCCGCCAGACTCCAGGCAAGGGGCTGGAGTGGGTGGC13010.1460206292018811No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12910.144898256955902No Hit
ATATTACTGTGCGAGAGGGGGGTCAAATTCTTTGGTGTTAATTCCTATTT12630.14175561466716052No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12530.14063324242118141No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12090.13569480453887336No Hit
ATATTACTGTCAACAGTATTATGATACCCCGCTCACTTTCGGCGGCGGGA11920.13378677172070888No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11400.12795043604161754No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10960.12301199815930952No Hit
TCTCTATGCAGCATCCAGATTGCAAAGTGGGGTCCCATCCAGGTTCAGTG10920.12256304926091788No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10840.1216651514641346No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGACATTAGCAA10600.11897145807378474No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10570.11863474639999103No Hit
TGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10430.11706342525562027No Hit
GTCTAAATAATATTTATTAATTCCATCATATGCTATAACTGCCACCCACT10360.11627776468343491No Hit
CAGTAATATACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATA10240.11493091798826No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.11459420631446625No Hit
CTCCCTGGTCTGTGCTGATACCAGGCTAAAGAATTGCTAATGTCCTGACT10110.11347183406848717No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9970.11190051292411642No Hit
GTGCTGATACCAGGCTAAAGAATTGCTAATGTCCTGACTCGCCCGGCAAG9590.10763549838939583No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9560.10729878671560208No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9360.1050540422236439No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9350.10494180499904598No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9300.10438061887605643No Hit
TATCAGCACAGACCAGGGAGAGCCCCTAAACTCCTTCTCTATGCAGCATC9260.10393166997766479No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9060.10168692548570658No Hit
CTCCAGCCCCTTGCCTGGAGTCTGGCGGACCCAGTGCATAGCATAGTCAC9000.10101350213811912No Hit
CAGTAATATGTTGCAAAATCTTCAGGGAGCAGGCTGCTGATGGTGAGAGT8970.1006767904643254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16200.060.9152681
ATCAACG17300.054.9417083
TCAACGC17600.053.5870444
CAACGCA18050.052.2510835
TATCAAC18950.050.917832
AACGCAG19350.048.740676
TATACAG17750.045.427715
CAGCCGT17100.044.628369
CGCAGAG21550.043.7648248
ATACAGC18700.041.579896
AATATAC18500.040.8687973
TAATATA19650.039.5763322
GAATACG2550.039.526569
ATATACA20750.038.8598484
ATACCCC17950.036.899859
GCAGAGT25800.036.8345539
TTATGAT18450.036.6802834
GTATTAT19550.036.4761541
GTACATG21400.036.3521921
TATGATA18350.035.7031485
Produced by FastQC (version 0.11.5)