FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662764
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATACTACGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGA56030.33696904671979905No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTATTGATTTGCAGATACA45960.27640723518190197No Hit
GTATATTACTGCGCGAGAGATGACTACACTGACTACTGGGAGGACAACTA43670.2626349860834129No Hit
GTATATGGAACTACCACTACTACTAATGTATGAAACCCACTCCAGCCCCT39670.23857865578037532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33440.20111092133339425No Hit
ATATACAGCCGTGTCCTCGTCTCTCAGGCTATTGATTTGCAGATACAGTG27530.16556769331065624No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27240.163823609363686No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26110.15702769605307787No Hit
GCTGTATATTACTGCGCGAGAGATGACTACACTGACTACTGGGAGGACAA25690.15450178137125894No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24620.1480667130151964No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22430.13489587217428328No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22150.13321192905307067No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21050.12659643821973532No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20920.12581460748488663No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC20850.12539362170458346No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19700.11847742674246016No Hit
GTGTAGTCATCTCTCGCGCAGTAATATACAGCCGTGTCCTCGTCTCTCAG19210.11553052628033803No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19120.1149892588485197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18930.11384658315912541No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17940.10789264140912361No Hit
CTCCAGAGACAATGCCAAGCAGTCACTGTATCTGCAAATCAATAGCCTGA17380.10452475516669833No Hit
GTATGATAAGTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGA17220.10356250195457684No Hit
CATTAGTAGTAGTGGTAGTTCCATATACTACGCAGACTCTGTGAGGGGCC17110.1029009528712433No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA17060.10260024874245532No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC16920.10175827718184902No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16770.1008561647954851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.1000141932348788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)