FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436963
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40450.16598528578398605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53350.071.293461
TCAACGC56250.065.285934
ATCAACG59500.061.8409083
CAACGCA60250.060.950345
AACGCAG62400.058.734896
GGTATCA26800.055.6398241
TATCAAC69700.053.2042542
ACGCAGA73950.049.557227
CGCAGAG79250.046.2429858
GCAGAGT93800.039.376939
GTACATG77600.029.7056061
ACATGGG74800.027.4356443
TATACCG2405.640686E-827.0018965
TACATGG82200.026.016962
CAGAGTA85250.024.5768369
GTATTAG15200.019.4307881
CATGGGG50800.018.5686154
AGAGTAC78750.018.45006810-14
CATGGGA39550.018.0243914
TATACAG20850.017.612755
Produced by FastQC (version 0.11.5)