FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2436963
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.1475607138885572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51300.073.553031
TCAACGC56400.065.4908144
ATCAACG60150.061.288133
CAACGCA61500.059.9427875
AACGCAG64750.057.4900746
TATCAAC66850.055.68412
GGTATCA26250.050.2005421
ACGCAGA74000.049.8173187
CGCAGAG78950.046.7850768
GCAGAGT91300.040.2946789
GTACATG74650.027.3952431
CAGAGTA84800.026.148849
ACATGGG74650.025.4633883
TACATGG79200.024.727822
CATGGGA35300.019.785144
GTCTAGG10900.019.1583541
AGAGTAC77150.018.570510-14
GTATTAA19400.018.5590151
GTATACG4003.8325743E-718.0022451
TATACCG2851.7227998E-417.68465
Produced by FastQC (version 0.11.5)