FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754844
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCTACACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG72710.4143388244197205No Hit
GTAATATAAAGCCGTGTCCTCAGTTCTCAGGCTGTTCATCTGCAGATACA66440.37860915272240725No Hit
TTATATTACTGTGCGAGCCTTCTAAATTACGTCCACGAAGTTGAGGACTA64430.3671551431352303No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC40650.23164452224813145No Hit
GTGTAGGTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA39350.22423645634597722No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA39230.22355263487808605No Hit
ATATAAAGCCGTGTCCTCAGTTCTCAGGCTGTTCATCTGCAGATACAGTG36500.20799569648356206No Hit
GCTTTATATTACTGTGCGAGCCTTCTAAATTACGTCCACGAAGTTGAGGA34460.1963707315294123No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG33590.19141302588720135No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33300.18976045733979774No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31950.18206746582602215No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31900.18178254021440082No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT30500.17380462308900393No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC29920.17049948599419665No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCCAAGTAGTCAGG29120.16594067620825553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28920.16480097376177028No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28040.15978628299723507No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27760.1581906995721557No Hit
ATATTACTGTGCGAGCCTTCTAAATTACGTCCACGAAGTTGAGGACTACT25870.14742051145286988No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25040.142690746299956No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24200.13790399602471787No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCCA23610.1345418738075863No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23410.13340217136110105No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23260.1325473945262371No Hit
CAGTAATATAAAGCCGTGTCCTCAGTTCTCAGGCTGTTCATCTGCAGATA22980.1309518111011577No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22670.12918527230910554No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA22430.12781762937332322No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21260.12115037006138436No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20440.11647759003079476No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC20250.11539487270663376No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19590.11163385463323235No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19540.11134892902161103No Hit
ATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGTGT18730.10673313411334569No Hit
CTGTATCTGCAGATGAACAGCCTGAGAACTGAGGACACGGCTTTATATTA18630.10616328289010306No Hit
ACACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGTTG18620.10610629776777879No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18270.10411181848642957No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18230.1038838779971325No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT17750.10114859212556786No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17680.10074969626929801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13700.074.145911
TCAACGC41900.065.818894
ATCAACG42250.065.273653
GTATCAA44250.063.4950681
TATCAAC44050.063.260252
CAACGCA45300.060.8736425
AACGCAG46750.059.139596
CGCAGAG50750.054.336478
GAATACG8950.046.658819
GTGCTAG9800.044.842771
GCAGAGT62050.044.3251579
GCTAGAG11350.038.0645983
GTGTAGA9750.036.944861
CTAGAGA12750.034.4497034
CAGCGCT9250.034.2483529
GTACCTA36550.033.3110281
TCTACAC22350.033.1837583
TACATGG46450.032.8636632
GTACATG47050.032.7674641
TATTGAG15600.032.307415
Produced by FastQC (version 0.11.5)