FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1754844
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACCTACACTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG62820.35798053844102384No Hit
GTAATATAAAGCCGTGTCCTCAGTTCTCAGGCTGTTCATCTGCAGATACA59760.3405430910097991No Hit
TTATATTACTGTGCGAGCCTTCTAAATTACGTCCACGAAGTTGAGGACTA57310.32658173604035456No Hit
GTGTAGGTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA38620.22007654241630598No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA36500.20799569648356206No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35730.20360784206459376No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33750.19232478784438958No Hit
ATATAAAGCCGTGTCCTCAGTTCTCAGGCTGTTCATCTGCAGATACAGTG32840.18713914171288162No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG31210.17785056677402664No Hit
GCTTTATATTACTGTGCGAGCCTTCTAAATTACGTCCACGAAGTTGAGGA29750.16953073891468415No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29520.16822008110122608No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT27660.15762084834891307No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26740.15237821709508081No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26040.14838925853238236No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25300.14417235948038687No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25070.1428617016669288No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCCAAGTAGTCAGG24480.13949957944979724No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24400.13904369847120315No Hit
ATATTACTGTGCGAGCCTTCTAAATTACGTCCACGAAGTTGAGGACTACT23750.135339665520126No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22730.1295271830430511No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAGCCA22380.1275327037617019No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21870.12462646252316444No Hit
CAGTAATATAAAGCCGTGTCCTCAGTTCTCAGGCTGTTCATCTGCAGATA21670.12348676007667918No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC21620.12320183446505786No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21010.11972574200327779No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA20080.11442612562712128No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20070.114369140504797No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19400.11055113730907135No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19030.10844268778307359No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18030.10274417555064723No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17850.1017184433488105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12200.081.43941
GTATCAA39000.066.4589161
ATCAACG38450.066.289843
TCAACGC38950.065.623734
TATCAAC40600.063.1341062
CAACGCA44600.057.471855
AACGCAG46100.055.6018336
CGCAGAG47400.053.7715538
GCAGAGT57700.044.1690449
GAATACG8700.043.030339
CAGCGCT8550.038.73319
GTGCTAG10400.037.3831371
GTACATG44200.036.6501351
TACATGG42350.036.2131462
CTAGAGA12850.033.6192634
GTACCTA38050.031.9777071
TATTGAG15650.031.284895
TACCTAC39800.030.211582
ACACTAT40350.030.1566587
AAGCCGT35800.029.7624879
Produced by FastQC (version 0.11.5)