FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190536
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36160.3037287406680688No Hit
GCTCTACAGTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA35720.3000329263457804No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28650.2406479098490092No Hit
CTGTAGAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT28060.23569215882594058No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21730.18252283005301814No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18640.15656813401694697No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18500.15539219309621885No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18420.15472022685580278No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18400.15455223529569873No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18300.15371227749517866No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15700.131873374681656No Hit
GTAATACACGGCCGTGTCGTCAGATATCAGGCTGCTCAGGTCCATGTAGA14720.123641788236559No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14290.12002996969432256No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14070.11818206253317834No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14060.11809806675312633No Hit
GTCTACATGGACCTGAGCAGCCTGATATCTGACGACACGGCCGTGTATTA13580.11406626931062983No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAAGCCCTGAGCTCCTGAT13170.11062244232849741No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC12890.10827056048704113No Hit
GTCTCCATCCTTCCTGTCTGCGTTTGTAGGAGACAGAGTCACCATCACTT12680.10650664910594893No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA12640.10617066598574086No Hit
CATGTAGACTGTGCCCGTGGACGTGTCCCTGGTCATGGTGACTCTGCCCT12340.10365079258418057No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)