FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190536
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30860.25921097724050346No Hit
GCTCTACAGTTACCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA30010.25207133593608255No Hit
CTGTAGAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT24010.20167386790487643No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22860.1920143531988953No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16970.1425408387482613No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16670.14002096534670097No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14870.12490172493733916No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14740.12380977979666301No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14090.11835005409328236No Hit
GTAATACACGGCCGTGTCGTCAGATATCAGGCTGCTCAGGTCCATGTAGA13130.11028645920828939No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12850.1079345773668331No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12560.1054986997453248No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12490.10491072928496073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19550.038.2943953
CTAGCGT1157.5011685E-637.5579644
TCAACGC20000.037.4327744
GTATCAA23050.036.266881
TATCAAC23050.034.6657562
GAATACG4100.033.359419
CAACGCA22700.032.6632965
GTACATG37000.030.9683821
CGCAGAG24750.030.5395588
ACATGGG39750.027.7078573
TCTAGCG1658.837244E-526.1767643
GTGCTAG7050.025.554891
TAGTACC3109.331416E-1025.5434534
TACATGG44700.025.2837092
GTAGAGC20500.024.931773
ATACACG9500.023.4902024
AACGCAG32400.023.3288296
ACGCAGA32750.023.2993187
TGTAGAG27050.022.6204012
TACACGG9900.022.5411015
Produced by FastQC (version 0.11.5)