FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091020
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12730.1166798042199043No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12220.11200527946325456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC22450.065.517164
ATCAACG23550.062.4569133
CAACGCA24650.059.669795
GTATCAA26000.058.7900471
TATCAAC25350.058.590952
AACGCAG26200.056.1319776
CGCAGAG29550.049.948248
ACGCAGA35000.042.4073877
GCAGAGT35200.041.9290589
GGTATCA13700.035.261071
TACGCAA1551.5379428E-632.5393377
GTACATG27800.026.7136781
TACATGG29450.023.7480352
CAGAGTA36250.023.6343069
ACATGGG30550.022.8929523
GTATAAC4557.2759576E-1222.1849231
CTATACT7050.021.4768924
ACCGTCC2404.6030706E-520.9995788
CTCTACG2104.4781493E-420.6002862
GACCGTC2206.1558594E-419.650387
Produced by FastQC (version 0.11.5)