FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091020
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC13550.055.8584564
ATCAACG14550.051.5239683
CAACGCA17100.043.842585
TATCAAC17200.043.5896872
GTATCAA19100.042.6700861
AACGCAG18400.041.1009066
CGCAGAG19050.039.320438
GTACATG29800.030.9793361
GCAGAGT25100.029.8427989
ACGCAGA26350.029.2471187
TACATGG29600.028.7388322
ACATGGG29850.027.2880843
GTATTAG6250.025.3875681
TATACTG6400.022.5272875
CCGACCG3554.451067E-922.3174329
GGTATCA12000.022.2381631
GTATATA8200.021.9889561
CAGAGTA26650.021.8911069
TATCCCG1650.00268864321.8446435
ACCGACC4007.4942363E-1021.6073348
Produced by FastQC (version 0.11.5)