FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057056
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTTCGGCGGTCACAGAGCTCAGCTTCAGGGAGA39480.37349014621741894No Hit
GTGTATTACTGTGCGACCCATCCGCGAAGTGGTTCGGGGAATTACTATGA30050.2842801138255684No Hit
GTCTCTGGCGGCTCCATCAGCAGTCGTAGTCACTACTGGGGGTGGTTCCG17910.16943283988738533No Hit
GTATTACTGTGCGACCCATCCGCGAAGTGGTTCGGGGAATTACTATGATT16300.1542018587473133No Hit
CAGTAATACACAGCCGTGTCTTCGGCGGTCACAGAGCTCAGCTTCAGGGA13640.12903762903762905No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12680.1199558017739836No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12280.11617170708079799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.11059016740834922No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11500.10879272242908607No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11310.10699527744982291No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11280.10671147034783399No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11200.10595465140919687No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11100.10500862773590047No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11010.1041572064299337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.068.841791
GTATCAA20650.059.0049931
ATCAACG21000.054.9238663
TCAACGC21100.054.321924
CAACGCA21750.053.3613785
AACGCAG22650.051.5568856
TATCAAC22750.050.698952
ACGCAGA25350.046.325847
CGCAGAG26000.045.1356438
GCAGAGT31650.037.0782559
GAATACG5050.035.641349
GTATTAG5250.032.9590031
GTAATAC18650.032.8595921
CAGCCGT18550.032.6017389
GTGCTAG6750.030.9753591
ACAGCCG21350.029.3377488
CAGAGTA31300.028.9822169
AATACAC22650.027.6892683
GTACATG24800.027.3273981
TACATGG24700.026.2667272
Produced by FastQC (version 0.11.5)