FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636393
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG14430.2267466801174746No Hit
ATATATTACTGTGCGAGAGGGGGGTGGATGTATTCCTACGGTATGGACGT14280.22438964602061934No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8820.1385936048950884Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8330.13089396017869462No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7430.11675175559756315Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGGGGCTGCGGTAAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA7000.10999492451991143No Hit
TTATTACTGCATGCAAAATACAGACTGGCCCCCCACCTTCGGCCAAGGGA6400.10056678813249045No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)