FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738062
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9980.13521899244236935No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG9670.13101880329836788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8100.055.1714481
TCAACGC8200.054.4247864
CAACGCA8400.053.1289565
ATCAACG8500.052.503913
AACGCAG8800.050.714016
TATCAAC9150.048.7741242
ACGCAGA9400.047.476947
GGTATCA5300.036.7194021
GCAGAGT14250.031.3181239
GAATACG1703.1835625E-629.6393059
CGCAGAG15350.028.6048978
GTACTAG1804.915586E-628.0306531
CCCGTTG1856.1472074E-627.2361168
CATAGGT6600.025.0843453
GTACATG17450.024.783551
TACACCG1751.3180116E-424.6792585
ACATGGG16050.024.6664433
CCGTTGA2051.3634757E-524.5789369
GTATTAG4400.024.5723291
CCCCGTT2452.034105E-623.5040557
Produced by FastQC (version 0.11.5)