FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866301
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATAACCCGTCCCTCATGGGTCGAGTCACCATATCACTGGACACGTCC20470.23629200474200077No Hit
CTACTGGACCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTG17330.20004594246110763No Hit
GAAGTACACAGCCGTGTCCGCGGCGGTCACAGAGTTCACCTCCAGGGAGA16790.19381254321534894No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13440.15514238122777188No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13400.15468064795030825No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13140.15167938164679481No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12280.14175211618132727No Hit
GTCCAAGAGTCCCTCTGCTCTCGCACAGAAGTACACAGCCGTGTCCGCGG12190.14071321630703418No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11440.1320557173545915No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11440.1320557173545915No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10980.12674578466376005No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10970.12663035134439415No Hit
TCACTGGACACGTCCAGGAACCAGTTCTCCCTGGAGGTGAACTCTGTGAC10880.12559145147010103No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10790.12455255159580791No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10770.12432168495707613No Hit
CCATATCACTGGACACGTCCAGGAACCAGTTCTCCCTGGAGGTGAACTCT10510.12132041865356269No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10470.12085868537609908No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10460.12074325205673317No Hit
CTCTTGGACCACGGTGACCACGCCCGCAACTGGGGCCAGGGAACCCTGGT10280.11866545230814693No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA9980.11520245272716989No Hit
GTGTACTTCTGTGCGAGAGCAGAGGGACTCTTGGACCACGGTGACCACGC9920.11450985281097448No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9840.11358638625604726No Hit
GGTTATAGTTGATTCTTCCAGTATCATGGATTTCTCCAATCCACTCCAGC9770.11277835302048594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.11150858650746102No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9630.11116228654936332No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9620.11104685322999741No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG9580.11058511995253383No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG8980.1036591207905797No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8790.10146588772262759No Hit
GTACACAGCCGTGTCCGCGGCGGTCACAGAGTTCACCTCCAGGGAGAACT8720.10065785448706627No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8720.10065785448706627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2300.037.646511
TCAACGC34500.029.2147084
GAATACG1000.006216865528.8623281
CAACGCA35050.028.5508735
AACGCAG35400.028.065226
ACGCAGA37350.026.5999687
CGCAGAG37850.026.248588
ATCAACG39300.025.6465023
TATCAAC41350.024.5647322
GCAGAGT42000.023.8263849
TATATAC6150.023.4124853
CATGGGG15200.022.7347554
ATACGAG2404.6042085E-520.9980723
GTACATG35900.020.7020851
TACATGG36500.020.328852
CTATAGA5353.6379788E-1220.1850624
TATAGGG2506.2737E-520.1709562
GTAGTAC2506.304432E-520.158153
GACTCGG8100.019.553767
GAGTTTA6750.019.241551
Produced by FastQC (version 0.11.5)