FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460725
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.12425336733471393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29300.064.4668661
ATCAACG33400.056.113873
TCAACGC33650.055.6969764
CAACGCA35150.053.1150675
AACGCAG38100.049.1832586
TATCAAC38850.048.0564962
CGCAGAG41050.045.424958
GGTATCA16350.044.976461
ACGCAGA42000.044.7663657
GCAGAGT51700.036.2068379
GTACATG37050.026.8530581
TACATGG38050.025.9542962
TACCGTC1959.203748E-625.8597327
ACATGGG38800.024.709463
ATACCGT2752.0857078E-723.5873266
CAGAGTA53550.021.1080469
GTATAGA8950.020.1381631
CATGGGG27150.019.1163944
CTAGTAC3703.5924386E-617.5341283
TACACCG3855.2456617E-616.8509795
Produced by FastQC (version 0.11.5)