FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460725
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21950.046.019441
TCAACGC20650.045.3975564
ATCAACG21350.043.909113
CAACGCA24850.037.728625
CGCAGAG25100.037.302888
ACGCAGA25250.036.79737
GTACCGT800.002103603736.012866
AACGCAG26750.035.0031556
TATCAAC28150.034.328162
TACATGG38550.027.8731442
GTACATG38750.027.743571
TACACGG2156.4158485E-726.8352385
ACATGGG40150.025.863433
GCAGAGT37200.025.3638959
TACCGTC2151.96372E-523.4502337
GTATAAT14050.023.1091521
GGTATCA15600.022.6631011
GTATATA17250.019.658781
CATGGGG27150.019.1236954
GTGTATA14900.018.4011571
Produced by FastQC (version 0.11.5)