FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645474
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17230.10471146915721548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC24200.056.6058854
ATCAACG24600.055.9785423
CGCAGAG28000.048.6039858
ACGCAGA28950.047.2577637
GTATCAA33150.044.395061
CAACGCA31000.044.186425
AACGCAG33850.040.6283876
TATCAAC37750.038.1976282
GCAGAGT36900.036.687059
GTACATG47500.032.0462231
TACATGG48150.030.3964672
ACATGGG49050.029.9857233
TCCGCTA6150.026.9290358
ATCCGCT6950.025.9014037
GGTATCA20500.025.689581
AAATCCG7400.025.3301945
AATCCGC6900.025.044776
GAATACG4051.8189894E-1224.8916859
CCGCTAC6900.024.0026999
CATGGGG29200.023.950374
Produced by FastQC (version 0.11.5)