FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1053356
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCA17240.16366736412001262No Hit
ATAATAGACGGCCGTGTCCGCGGCGGTCAGAGAGGTCAGTTTGAGGGAGA15520.14733860157439652No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15500.14714873224247071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13110.12445934707734138No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11830.11230770983409219No Hit
GTCTATTATTGTGCGAGTGAGTGGGGCATAGACAAACGTTCTACGGGCTG10990.1043331978932099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18500.063.5006141
TCAACGC21950.051.5498244
CAACGCA22300.050.738345
ATCAACG22400.050.5142253
AACGCAG22850.049.5170636
TATCAAC25350.045.2044532
GGTATCA10450.044.1393361
CGCAGAG26750.042.528778
ACGCAGA30600.036.9601757
GCAGAGT32400.035.1091549
CAGCGCT6100.027.1460299
GTACACG7300.026.6552245
TACACGG7550.025.77266
CAGAGTA31150.025.6550719
GTACATG24050.024.273481
TACATGG24100.023.6250152
GTATATA7150.023.1837581
GCAGCGC8700.023.1733238
GTATAGG4750.022.7593441
ATCGACC1600.002263969522.5008838
Produced by FastQC (version 0.11.5)