FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830431
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGATAGCCGTGTCCTCACCTCTCAGGCTGTTCATTTGCAGATACA32650.39316933014302213No Hit
ATCTACTACTGTGCGAAAGATGATGAGGACCTTGCAGTGGGAAGAGTTGC28590.34427905509307816No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTAGAGTCTGGGGGAGGCG11210.13499014367238218No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.1288487544419705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14500.072.0579451
ATCAACG16550.061.3870543
TCAACGC16650.061.0183644
CAACGCA16900.060.1157265
AACGCAG17050.059.160686
TATCAAC18300.055.5167122
GGTATCA6750.054.4437751
ACGCAGA19500.051.7120937
CGCAGAG20550.049.0491878
ATAGCCG11600.047.7912458
TAGATAG13800.042.8144575
AGATAGC13400.041.401446
GATAGCC13800.041.233177
GCAGAGT24950.040.9763649
GTAGTAG14850.039.789571
GTAGATA14550.039.1218874
AGTAGAT15150.037.5725063
TAGTAGA17600.032.7516372
TAGCCGT18450.030.0476119
CTGTGCG13300.028.149289
Produced by FastQC (version 0.11.5)