FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163987
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT60970.28174845782345276No Hit
ATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAAGGA41340.19103626777794874No Hit
GACTTATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCA40290.18618411293598344No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37980.17550937228365976No Hit
CACTATCACCAGTCTGCAGCCTGAAGATTTCGCAACTTACTACTGTCAAC32650.1508789100858739No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31740.1466737092228373No Hit
CTATAAGTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGCTGCAGACT31030.14339272832969882No Hit
GTAATACACGGCCGTGTCCTCCGATGTCAGGCTGCCCAGACTCATGTACA29720.1373390875268659No Hit
GGGTACTATAAGTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGCTGC26130.12074933906719404No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25800.11922437611686207No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGTACAGATTTCACT25730.11890089912739772No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25090.11594339522372361No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24580.11358663430048332No Hit
GTGTATTACTGTGCGAGAGGGTTCGAGGGCCTTTTCGGGAGGGCCTCGGA22980.10619287454129808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22190.10254220566020036No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21940.10138693069782767No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC44000.062.507854
ATCAACG44800.061.5537833
CAACGCA48050.057.538935
AACGCAG50750.054.6208726
GTATCAA58000.051.3909031
CGCAGAG57800.047.9586458
ACGCAGA60600.045.861547
TATCAAC70150.041.5682072
GCAGAGT66650.041.4777159
TACATGG64500.031.0326612
ACATGGG64500.030.586153
GTACATG80550.028.2446021
ATATCGC1959.243784E-625.8462416
GGTATCA38550.025.7732141
GTATACG1400.001041293125.7131861
ATACACG20100.025.4323354
CATGGGA33750.025.3861664
CAGAGTA69250.024.9502689
CATGGGG41050.024.7303284
GTAATAC23900.023.7981431
Produced by FastQC (version 0.11.5)