FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896614
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.19550630755651915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40000.080.855471
ATCAACG46950.067.632323
TCAACGC47300.067.131864
CAACGCA51100.062.4198385
TATCAAC53450.060.215882
GGTATCA17800.059.0820961
AACGCAG54000.058.800996
ACGCAGA60100.052.7102557
CGCAGAG61600.051.4267278
GCAGAGT74350.042.7045829
GTACATG49600.027.4475231
TACATGG50200.025.961282
ACATGGG50800.024.5207443
CAGAGTA75300.024.0947559
ATACCGA2806.4701853E-620.5717756
TAGACGG4001.7682396E-819.8008564
TATACTG15250.018.8855655
TATACCG1950.007120831418.461855
TATACGG2000.00824007718.0003055
AGAGTAC66600.017.92368110-14
Produced by FastQC (version 0.11.5)