FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG23280.48692538412305325No Hit
ATCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA20940.4379818532447051No Hit
GTTTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCGGGGGGTTTT15870.33193753634161743No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA15710.3285909701277133No Hit
GTATGTGATACTACCATCCCCACTAATAAGAGAGACCCACTCCAGACCCT13630.2850856093469594No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11160.23342299341981418No Hit
TTGTATTACTGTGCAAAAGGTTCTTACGATTTTTGGAGTGCTTACCATGC10920.22840314409895796No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9120.19075427419253632No Hit
CTCTTATTAGTGGGGATGGTAGTATCACATACTATGCAGACTCTGTGAAG8310.17381228273464658No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT7320.15310540428611466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.15205960234426963No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT6810.14243822447929522No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACCCCCCGACCACTC6500.13595425243985593No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6420.13428096933290387No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6280.1313527238957377No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGGTTCTTAC6250.1307252427306307No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC6170.12905195962367863No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6150.1286336388469406No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6150.1286336388469406No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA5910.1236137895260844No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCA5830.12194050641913233No Hit
GTATTACTGTGCAAAAGGTTCTTACGATTTTTGGAGTGCTTACCATGCCT5800.1213130252540253No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5790.12110386486565629No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC5680.11880310059359718No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5430.11357409088437195No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA5340.11169164738905088No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC5270.11022752467046781No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC5080.10625347729145664No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5020.10499851496124257No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG5000.10458019418450457No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC4910.10269775068918348No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4870.10186110913570745No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT4840.10123362797060043No Hit
GGTCTGGAGTGGGTCTCTCTTATTAGTGGGGATGGTAGTATCACATACTA4830.10102446758223141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG400.00621818553.997069
CTATAAC803.8766317E-545.021091
GTATCAA13050.041.9506871
TCAACGC12800.041.0602654
ATCAACG12850.040.9047743
CAACGCA13300.039.5166445
AACGCAG13400.039.2217456
TATCAAC13750.038.2273672
ACGCAGA14650.035.875187
CGCAGAG14800.035.5115788
CATACTA11600.035.3773845
ACATACT12300.035.1200374
GAATACG1459.1728543E-734.756739
ATACTAT11950.034.341236
TACTATG12050.033.458767
CACATAC12700.033.4504973
ACTATGC12000.032.9982038
TCACATA12400.032.5177572
GCAGAGT16600.032.094649
GTACATG15150.031.3810391
Produced by FastQC (version 0.11.5)