FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478102
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCACATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA19500.4078627573195678No Hit
GTAATAAACAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG16050.33570242333225964No Hit
GTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATACA15650.3273360077974993No Hit
GTTTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCGGGGGGTTTT15560.3254535643021782No Hit
GTATGTGATACTACCATCCCCACTAATAAGAGAGACCCACTCCAGACCCT13000.27190850487971185No Hit
TTGTATTACTGTGCAAAAGGTTCTTACGATTTTTGGAGTGCTTACCATGC11810.24701841866379978No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10280.21501687924334137No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9710.20309473710630788No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT8740.18280617943451397No Hit
CTCTTATTAGTGGGGATGGTAGTATCACATACTATGCAGACTCTGTGAAG8630.18050541516245489No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACCCCCCGACCACTC7960.16649166914173125No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGGGAGTTTTTGCTGTTGTCTCT7120.1489221965187345No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7040.14724891341178242No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAAAACCCC6970.14578479069319936No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6360.13302600700268982No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6190.12947028040041664No Hit
GTATTACTGTGCAAAAGGTTCTTACGATTTTTGGAGTGCTTACCATGCCT6050.12654203496325053No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5780.12089470447728728No Hit
CTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCA5720.11963974214707321No Hit
CAGTAATACAAGGCGGTGTCCTCAGTTCTCAGACTGTTCATTTGCAGATA5710.1194305817587042No Hit
GTCTGAGAACTGAGGACACCGCCTTGTATTACTGTGCAAAAGGTTCTTAC5650.11817561942849014No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT5620.11754813826338312No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5490.114829053214586No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5390.11273744933089593No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG5280.11043668505883683No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5180.10834508117514671No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC5160.1079267603984087No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA5090.10646263767982564No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5020.10499851496124257No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4850.10144278835896943No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG4810.10060614680549339No Hit
GATTATTCCTATTGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTAGCTGG4790.10018782602875538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAGC605.09412E-448.0380061
GAATACG1851.8189894E-1242.808859
CTTAGAC951.0671755E-437.91683
GTGCTAG2550.036.7349471
GTATAGG1001.4413966E-436.0285071
GTCTATA1251.317884E-534.5873641
TAGACTC1051.922556E-434.305685
GTACATG15300.032.0253371
CTAGAGA2705.456968E-1232.0186354
CGTAGCA900.00374066232.018632
GACTATG950.00488610430.3175727
TACATGG15800.030.0934622
TGCTAGA3600.030.0174672
ATGGGAG6550.029.696675
GTCTAAA1703.154475E-629.6705341
CCTAGAC1753.959851E-628.816773
ATCAACG9750.028.8167693
AGGCGGT8650.028.2992829
TCAACGC10000.028.0963524
GTAATAC8700.027.331971
Produced by FastQC (version 0.11.5)