FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883553
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29260.33116292967145156No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC23860.27004605269859305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22270.2520505278121403No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA21390.2420907404536004No Hit
GGTACACACTGGCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAA21130.23914807600675905No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT20330.2300937238626319No Hit
GTACACACTGGCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA18930.21424860761040934No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC15670.17735212262309108No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15130.17124043492580523No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14690.1662605412465353No Hit
TTATTACTGCATGCAAGGTACACACTGGCCTCGTACGTTCGGCCAAGGGA13670.15471624226277314No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG13520.15301855123574928No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT13410.15177357781593181No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13190.14928363097629682No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12990.14702004294026505No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12690.14362466088621736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12530.1418137904573919No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG12320.13943702301955851No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA11850.1341175911348838No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11840.13400441173308222No Hit
TTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC11740.1328726177150663No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAA11660.13196718250065362No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT11640.13174082369705042No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA11540.13060902967903454No Hit
GTGTATTACTGTGCGAGAGAGGTCCCGCTATCATTATGGTTCGGGGACGG11320.12811908283939955No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA10950.12393144497274075No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTG10900.12336554796373278No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC10780.12200739514211371No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10650.12053606291869305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.11714068086464537No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10290.11646160445383583No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10110.11442437522140722No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10070.11397165761420085No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9730.11012355795294679No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC9490.10740725230970864No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9300.10525684367547843No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA9250.10469094666647048No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9240.10457776726466889No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9110.10310643504124824No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA9090.10288007623764504No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8920.10095602640701803No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8920.10095602640701803No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8890.10061648820161326No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8870.10039012939801009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)