FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883553
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28450.32199539812552275No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC23470.26563205602833107No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA22430.25386139824096576No Hit
ATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGT21610.24458068729323537No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20330.2300937238626319No Hit
GGTACACACTGGCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAA19710.22307660095093335No Hit
GTACACACTGGCCTCGTACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA18320.20734466410051236No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGCTGGATCCGGCAGCC14670.1660341824429321No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14350.16241244158528126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14310.16195972397807487No Hit
GTAATAGATATACCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGCCGGA13840.15664029209340016No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13730.15539531867358267No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13390.15154721901232865No Hit
TTATTACTGCATGCAAGGTACACACTGGCCTCGTACGTTCGGCCAAGGGA13280.15030224559251112No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13150.1488309133690905No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGA13010.14724640174386822No Hit
GGGTATATCTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAG12740.1441905578952253No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG12630.1429455844754078No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT12280.13898430541235218No Hit
GTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAA11560.1308353884826377No Hit
GTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAGGCCAATCT11550.13072220908083612No Hit
TTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGC11470.1298167738664234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11300.12789272403579638No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTG11110.12574231540156616No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA11070.12528959779435983No Hit
CTCGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCC10770.12189421574031213No Hit
GTGTATTACTGTGCGAGAGAGGTCCCGCTATCATTATGGTTCGGGGACGG10650.12053606291869305No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT10610.12008334531148669No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10540.11929108949887555No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10520.11906473069527239No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9530.107859969916915No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9420.1066149964970975No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA9410.10650181709529594No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9380.10616227888989116No Hit
CTGTATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC9300.10525684367547843No Hit
CTATTACAGTGGGAGCACCAACTACAACCCCTCCCTCAAGAGTCGAGTCA9100.10299325563944664No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9090.10288007623764504No Hit
GTTTATTACTGCATGCAAGGTACACACTGGCCTCGTACGTTCGGCCAAGG9050.1024273586304387No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9050.1024273586304387No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9030.1022009998268355No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9000.10186146162143074No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8930.10106920580881962No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8900.10072966760341485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG553.3282003E-452.3714835
ATCAACG12150.052.1589163
TCAACGC12250.051.73024
CAACGCA12850.049.314785
AACGCAG13650.046.4140246
TATCAAC14150.045.30072
GTATCAA17200.041.462161
CGCAGAG15400.041.1420338
GTACACG5800.036.0053945
ATACCGT1251.3270161E-534.557356
GCAGAGT19450.032.943499
GTACATG22850.031.8405341
ACGCAGA20050.031.5985767
GTATGTA9600.031.5154291
ACGGGGT6950.031.0767549
CAGCGCT4600.029.7368499
ACACGGG7050.029.6147527
TACATGG24000.029.4094032
GAATACG1753.9852494E-628.7977929
TATTACG1000.00628528728.7977927
Produced by FastQC (version 0.11.5)