FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738678
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG30280.40992150842450975No Hit
ACTGTACACTGGTACCAGCAGCTCCCACTAACGGCCCCCAAACTCCTCAT30090.40734934572303494No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAG29830.4038295441315431No Hit
GTATTAACTCCGATGTTGGAGCTGCTTCCAGAACAGGAGATGGTGACGCT27970.3786494250539477No Hit
GAGTTAATACTGTACACTGGTACCAGCAGCTCCCACTAACGGCCCCCAAA26600.3601027782064716No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24460.33113210356880807No Hit
GTAATATACGGCAGTGTCTTCAGCCTGAAGGCTACTGATCTGCAGATATG22560.30541047655406006No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21900.29647559559104236No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21540.2916020241566691No Hit
GTGTACAGTATTAACTCCGATGTTGGAGCTGCTTCCAGAACAGGAGATGG21180.2867284527222958No Hit
GATTATTACTGCGCGACATGGGATGACAACCTGAATGGTCTGGTGTTCGG20670.2798242265236003No Hit
GTAATAATCACCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG18350.2484167661687501No Hit
GTATATTACTGTGCGAGAGGTACCAGCAGTTGGAAGGCGAAGTACAACTG17990.24354319473437683No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16660.22553805582405323No Hit
GATTATTACTATGGATGAGGAGTTTGGGGGCCGTTAGTGGGAGCTGCTGG15700.2125418653323911No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15640.21172960342666225No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15370.20807442485088226No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14350.19426597245349123No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGCGTCAC12590.17043962321877734No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12590.17043962321877734No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC12430.16827359147016696No Hit
ATACTGTACACTGGTACCAGCAGCTCCCACTAACGGCCCCCAAACTCCTC12200.16515992083153958No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11670.15798494066426777No Hit
GGTACCAGCAGCTCCCACTAACGGCCCCCAAACTCCTCATCCATAGTAAT11660.15784956367997963No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11090.15013307557555525No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACCATTCAGG11070.14986232160697896No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10980.14864392874838564No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAGACCATTCAGGT10850.14688402795263972No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10840.14674865096835157No Hit
GCTCCACACCATGGACTGGACCTGGAAGATCCTCTTCTTGGTGGCAGCAG10510.14228121048684272No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10500.14214583350255458No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10480.14187507953397827No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT10320.1397090477853679No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT9750.13199255968094353No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9740.1318571826966554No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9700.1313156747595028No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9590.12982652793233318No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9580.129691150948045No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG9500.12860813507373983No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9410.1273897422151465No Hit
GTACACTGGTACCAGCAGCTCCCACTAACGGCCCCCAAACTCCTCATCCA9310.12603597237226505No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9290.12576521840368873No Hit
ATCCCATGTCGCGCAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAC9240.12508833348224802No Hit
ATATACGGCAGTGTCTTCAGCCTGAAGGCTACTGATCTGCAGATATGCCG9130.1235991866550784No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8980.12156853189075618No Hit
GCCGTATATTACTGTGCGAGAGGTACCAGCAGTTGGAAGGCGAAGTACAA8930.12089164696931545No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8920.12075626998502731No Hit
GTATTGGGGTTGATCCAACCCATCCACTCAAGTCCTTGTCCAGGGGCCTG8910.12062089300073917No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8840.11967325411072213No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8790.11899636918928139No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8770.1187256152207051No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8700.11777797633068807No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8520.11534119061350143No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8490.11493505966063697No Hit
GTACAGTATTAACTCCGATGTTGGAGCTGCTTCCAGAACAGGAGATGGTG8370.11331053584917922No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8360.11317515886489107No Hit
ACATGGGATGACAACCTGAATGGTCTGGTGTTCGGCGGAGGGACCAAGCT8350.1130397818806029No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8280.1120921429905859No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8100.10965535727339923No Hit
GTCTGAGGATGAGGCTGATTATTACTGCGCGACATGGGATGACAACCTGA8080.10938460330482294No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7790.10545867076046667No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7700.10424027790187335No Hit
ACACCAGACCATTCAGGTTGTCATCCCATGTCGCGCAGTAATAATCAGCC7600.10288650805899188No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT7520.10180349218468669No Hit
CTCCCAGGTACAGCTGGTGCAATCTGGGTCTGAGTTGAAGAAGCCTGGGG7470.10112660726324596No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7440.10072047631038152No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC259.630332E-486.4384463
GAATACG1950.059.07129
TAATACG450.00989051848.018114
GCGTGTC450.00990905647.9953547
ATCAACG11750.047.2039763
TCAACGC11700.046.7868774
AAATCCG952.0096213E-645.487765
AATCCGC952.015251E-645.4692846
GTATCAA12850.044.284291
CAACGCA12800.043.888585
AACGCAG13600.041.290126
TATCAAC13650.041.1611632
TATACGG8950.036.2123225
ATACGGC8750.036.202216
CGGCAGT9300.034.8353359
TACGGCA9100.034.809827
AGTACCC850.00283182833.8928385
GTGCTAG3450.033.4061621
AATATAC10800.033.3481643
GGTATCA7150.033.2455561
Produced by FastQC (version 0.11.5)