FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870910
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA56510.6488615356351403No Hit
GTATATTACTGTGCGAAAGAGACCTTGGACGTCTTATGGGGAGACCTCGG36300.41680541043276575No Hit
GTATAATAGTTACCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATATCA28260.32448817903112837No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24240.27832956333030967No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG22360.2567429470324144No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22170.2545613209172016No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20660.23722313442261544No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT20050.23021896636851108No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18600.21356971443662376No Hit
GCCGTATATTACTGTGCGAAAGAGACCTTGGACGTCTTATGGGGAGACCT18260.20966575191466397No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18210.20909163977908163No Hit
GTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAGCCCCT18030.2070248360909853No Hit
AGCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA17910.2056469669655877No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17510.20105406988092916No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17420.20002066803688096No Hit
CCCCATAAGACGTCCAAGGTCTCTTTCGCACAGTAATATACGGCCGTGTC17140.19680564007761997No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16480.18922735988793332No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA15790.18130461241689727No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14780.16970754727813436No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14630.1679852108713874No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT14450.16591840718329104No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGG13870.1592587064105361No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC13870.1592587064105361No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13570.15581403359704218No Hit
CCGTAGTAGTAGTAGTAACCGCCGAGGTCTCCCCATAAGACGTCCAAGGT13330.15305829534624704No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGG13210.15168042622084946No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT13160.15110631408526712No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12250.14065747321766886No Hit
CTTATGGGGAGACCTCGGCGGTTACTACTACTACTACGGTATGGACGTCT11940.13709797797705847No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11750.13491635186184567No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT11560.13273472574663284No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11400.1308975669127694No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC11390.13078274448565294No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA11240.12906040807890598No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC11200.1286011183704401No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT11190.12848629594332364No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11080.12722324924504252No Hit
ATATTACTGTGCGAAAGAGACCTTGGACGTCTTATGGGGAGACCTCGGCG10990.12618984740099437No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10980.1260750249738779No Hit
GCGTAGTATGTGCTACCACCACTACCACTAATAGCTGAGACCCACTCCAG10660.12240070730615105No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT10590.12159695031633579No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10560.1212524830349864No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10360.11895603449265711No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT10280.1180374550757254No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10270.11792263264860893No Hit
CAGTATAATAGTTACCCTCCGACTTTCGGCCCTGGGACCAAAGTGGATAT10120.11620029624186197No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10020.11505207197069732No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10020.11505207197069732No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9880.11344455799106681No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9820.11275562342836803No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC9560.10977024032333994No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9400.10793308148947653No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA9260.10632556750984601No Hit
GGGCTGGAGTGGGTCTCAGCTATTAGTGGTAGTGGTGGTAGCACATACTA9210.10575145537426371No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC9190.10552181052003078No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG8940.10265124984211918No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8930.10253642741500271No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8900.10219196013365331No Hit
CTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCG8850.10161784799807098No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8840.10150302557095452No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8780.10081409100825572No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8740.10035480129978987No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA8720.10012515644555695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.055.8425061
ATCAACG16700.050.442453
TCAACGC17350.048.54714
CAACGCA17900.047.055435
TATCAAC17950.046.929752
AACGCAG18550.045.4065866
GGTATCA8150.043.307671
GGCAGCG5350.043.0574387
CAGCGCT5350.041.711899
CGCAGAG21400.039.357198
GCAGCGC5600.038.5642788
GCAGAGT23850.035.917879
TATACTG10800.032.6624875
TAATAGT13000.032.1189654
TACTGTT10250.031.6038957
CTATTAT9350.030.815891
ATAATAG13350.030.7411693
TTATACT10800.030.6627434
AATAGTT13150.030.6576715
TACACGG10350.030.6047766
Produced by FastQC (version 0.11.5)