FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725263
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39620.14538046419740042No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC66050.061.593494
GTATCAA69550.060.148151
ATCAACG75300.054.6009673
CAACGCA75800.053.670855
AACGCAG77100.052.9526756
CGCAGAG83750.048.5761268
ACGCAGA83700.048.347067
TATCAAC87500.047.2349052
GGTATCA41750.044.322061
GCAGAGT99750.040.130389
GTACATG77250.030.2920151
TACATGG78800.027.8697342
ACATGGG80500.026.2078233
TAGACGG13450.025.16145
CAGAGTA99050.024.495539
GTAATAG18600.023.2263831
GACGGCC15450.022.8363577
CATGGGG52850.021.3901524
TTTCGCG7400.020.4314389
ATAGACG17350.019.9205364
Produced by FastQC (version 0.11.5)