FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706406
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGA58170.21493449245974183No Hit
GTAATACACAGCCGTGTCGTCGTCTCTCAGGCTATTCATTTGCAGATACA50090.18507940050384164No Hit
GTGTATTACTGTGCGAGAGATGACTACACTGACTACTGGGAAGACACCTA45900.1695976139574033No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT42940.15866060007256858No Hit
ACCATACATTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGA39150.1446567883754322No Hit
TTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGCT36100.13338723014950454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.12252411500713492No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29680.10966573381820761No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAGAATCTTCAGACTGCAGGC29430.10874199953739387No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29330.10837250582506838No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28790.10637723977851068No Hit
GTACTACCATACATTATGCAGACTCTGTGAGGGGCCGATTCACCATCTCC28410.10497316367167381No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC27160.1003544922676051No Hit
GTATAGCATGAACTGGGTCCGCCAGGCTCCAGGGAACGGTCTGGAGTGGG27150.10031754289637253No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)