FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1535720
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC69060.44969134998567445No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATCGGTGTTG50470.32864063761623213No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCTCAGCTCCATGTAGG45580.2967988956320162No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC42860.279087333628526No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA41690.27146875732555414No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC39380.25642695282994293No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT39300.25590602453572264No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGGCCAGAGATGGTCAGGGAGG39190.25518974813116974No Hit
GATTATTACTGCTGCTCATATTCAAGCAGCAACACCGATTGGGTGTTCGG36940.24053863985622376No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36560.23806423045867733No Hit
GTGTATTACTGTGCGAGAGATGGGGGTACACCTATGGCCCCTATTAAGTT34450.22432474669861693No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGAGTCCTCGGTGAAGGTCTCCTGCA33950.22106894485974005No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCCGAGGACACGGCCGTGTATTA32150.2093480582397833No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32140.20928294220300575No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA29650.1930690490453989No Hit
CCTATGGCCCCTATTAAGTTTGACTACTGGGGCCAGGGAACCATGGTCAC29640.19300393300862137No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCT29210.19020394342718724No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA29170.1899434792800771No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28440.18519000859531684No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28350.18460396426431902No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27620.17985049357955876No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC27590.17965514546922617No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27380.17828770869689786No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26580.17307842575469487No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26220.1707342484307035No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG25930.16884588336415493No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT25850.16832495506993464No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25480.16591566170916572No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC24990.1627249759070664No Hit
TCCCTATCTTTGGTACAGCAAAGTACGCACAGAAGTTCCAGGGTAGAGTC24780.1613575391347381No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATCGGTGTTGC24690.16077149480374026No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24440.15914359388430183No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23220.15119943739744224No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22860.1488552600734509No Hit
TCTCTGGCCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT22720.14794363555856535No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22660.14755293933790015No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22540.14677154689656968No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22440.14612038652879433No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22030.14345062902091527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.14253900450602974No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21660.14104133566014637No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21500.13999947907170576No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC21410.13941343474070794No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21140.13765530174771443No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT20940.13635298101216367No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20280.1320553225848462No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20070.1306878858125179No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT19660.12801812830463888No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG19410.12639022738520045No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG19410.12639022738520045No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19400.1263251113484229No Hit
GGCCATAGGTGTACCCCCATCTCTCGCACAGTAATACACGGCCGTGTCCT19180.12489255853931706No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18660.1215065246268851No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC18140.11812049071445316No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATA18010.11727398223634518No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG17860.11629724168468211No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17640.11486468887557627No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17610.11466934076524365No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17430.11349725210324799No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG17370.11310655588258275No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17360.11304143984580522No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17100.11134842288958925No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17080.11121819081603417No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTACCCT16920.11017633422759357No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16700.10874378141848774No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT16510.10750657671971453No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAACACCCAGGCAAGGCCCCCAA16240.10574844372672101No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG16080.10470658713828043No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTACCC15880.10340426640272966No Hit
GATTTATGGTGTCAGTAATCGTCCCTCAGGGGTTTCTAATCGCTTCTCTG15810.1029484541452869No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGT15770.10268798999817676No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG15730.10242752585106661No Hit
GTATTACTGTGCGAGAGATGGGGGTACACCTATGGCCCCTATTAAGTTTG15560.10132055322584846No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15510.10099497304196078No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)