FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827146
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTATAGTGGGAGCCCCTACTACAACCCGTCCCTCAAGAGTCAAGT64930.35536295402775697No Hit
CTATTATAGTGGGAGCCCCTACTACAACCCGTCCCTCAAGAGTCAAGTCG61880.33867025404647466No Hit
GTCTCTGGTGACTCCATCAGCAGAAGTAATTACTACTGGGGCTGGGTCCG39810.2178807823786386No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33850.18526160471029682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.17787303258743417No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31250.1710317621033021No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30780.16845944440126842No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT30700.16802160309028397No Hit
GTCTACTTCTGTGCGAGACATGCTTCGCATGTTGTGGCGGTGACTGCCGG30360.16616077751860006No Hit
ACTACAACCCGTCCCTCAAGAGTCAAGTCGCCATAGCCGTAGACACGTCC29270.16019518965643686No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC27220.14897550606246024No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG27100.14831874409598356No Hit
ATAATAGATAGCCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGA26920.14733360114626856No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26890.1471694106546494No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26200.14339302934740847No Hit
CTATAATAGATAGCCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCG25590.140054489351152No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25100.13737271132137224No Hit
GAAGTAGACAGCCGTGTCTGCGGCGGTCACAGAGTCCACCTCCAGGGAGA24530.134253091980608No Hit
ATTATAGTGGGAGCCCCTACTACAACCCGTCCCTCAAGAGTCAAGTCGCC24200.13244699657279713No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24170.13228280608117798No Hit
GCCGTAGACACGTCCAAGAACCAGTTCTCCCTGGAGGTGGACTCTGTGAC24110.13195442509793964No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21720.1188739159322791No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21280.1164657887218646No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19900.10891302610738277No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37200.078.2325741
ATCAACG44350.065.102523
TCAACGC44850.064.376754
TATCAAC46000.062.765612
CAACGCA47900.060.127295
AACGCAG49350.058.3606266
CGCAGAG56600.050.8837138
ACGCAGA63650.045.247737
GCAGAGT65750.043.8025559
GGTATCA30700.040.1242521
TATATCG2053.1468517E-1035.1231355
CAGCGCT9900.032.7274789
GTACTAG3101.8189894E-1230.2086181
GTACACG16550.029.5840785
TACACGG17850.027.8328696
GTACATG45600.027.8033431
TACATGG45750.027.22642
ACACGGG18050.027.1248257
GAATACG4000.027.000179
ACATGGG46450.026.5068113
Produced by FastQC (version 0.11.5)